FastQCFastQC Report
Wed 5 Oct 2016
example_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenameexample_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences247412
Sequences flagged as poor quality0
Sequence length35-301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA85663.4622411200750167No Hit
GGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCA81743.3038009474075634No Hit
GGACTACCGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCA74623.0160218582768823No Hit
GGACTACTAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCA63082.549593390781369No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA60852.4594603333710574No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGA44281.789727256559908No Hit
GGACTACAGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCA43921.775176628457795No Hit
GGACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCA40891.6527088419316767No Hit
GGACTACAAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCA36551.4772929364784246No Hit
GGACTACCAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCA36021.4558711784392027No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA34031.375438539763633No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA32801.32572389374808No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGA32281.3047063198228057No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA31981.292580796404378No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA31121.2578209626048857No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA23090.9332611191049747No Hit
GGACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCA21600.8730376861267845No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA20720.8374694840993969No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA19580.7913924951093723No Hit
GGACTACCAGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTAA18970.7667372641585695No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGA18850.7618870547911984No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGA18210.7360192714985531No Hit
GGACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCA18090.7311690621311819No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGA16460.6652870515577255No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA14750.5961715680726885No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA14360.5804083876287326No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCA14320.5787916511729423No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA14130.5711121530079382No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA14000.5658577595266195No Hit
GGACTACCCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCA13020.5262477163597562No Hit
GGACTACTAGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTAA12840.5189724023086997No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCA12390.5007841171810583No Hit
GGACTACCAGGGTATCTAATCCTGTTCGCTCCCCCGGCTTTCGCACTTCA12310.4975506442694776No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA12210.4935088031300018No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA12050.4870418573068404No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA11980.48421256850920735No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA11830.4781498067999935No Hit
GGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCA11590.4684493880652515No Hit
GGACTACCCGGGTATCTAATCCGGTTCGCTCCCCACGCTTTCGTGCCTTA11450.46279081046998527No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGA10970.4433899730005012No Hit
GGACTACTAGGGTATCTAATCCTGTTCGCTCCCCCGGCTTTCGCACTTCA10660.43086026546812606No Hit
GGACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCA10200.41226779622653714No Hit
GGACTACTCGGGTATCTAATCCGGTTCGCTCCCCACGCTTTCGTGCCTTA10160.4106510597707468No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCA9970.4029715616057426No Hit
GGACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCA9290.37548704185730686No Hit
GGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCA9180.3710410166038834No Hit
GGACTACTAGGGTATCTAATCCGGTTCGCTCCCCACGCTTTCGTGCCTTA9110.36821172780625033No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA9020.364574070780722No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA8230.33264352577886275No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCA8010.3237514752720159No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA7950.3213263705883304No Hit
GGACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCA7950.3213263705883304No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGA7930.3205180023604352No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA7600.3071799266001649No Hit
GGACTACAAGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTAA7580.3063715583722697No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCCGGCTTTCGCACTTCA7530.3043506378025318No Hit
GGACTACACGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCA7340.29667113963752767No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA7340.29667113963752767No Hit
GGACTACAAGGGTTTCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCA7020.283737247991205No Hit
GGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTAA6930.28009959096567666No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA6680.2699949881169871No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCCGGCTTTCGCACTTCA6330.2558485441288216No Hit
GGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTAA6140.24816904596381742No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA6140.24816904596381742No Hit
GGACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA6090.24614812539407951No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA5960.24089373191276092No Hit
GGACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCA5950.24048954779881332No Hit
GGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCA5840.23604352254538988No Hit
GGACTACTGGGGTATCTAATCCTGTTCGCTCCCCCGGCTTTCGCACTTCA5840.23604352254538988No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGA5790.23402260197565192No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGA5790.23402260197565192No Hit
GGACTACCAGGGTATCTAATCCTGTTCGATACCCGCACCTTCGTGCTTAA5740.232001681405914No Hit
GGACTACAAGGGTATCTAATCCTGTTCGCTCCCCCGGCTTTCGCACTTCA5610.22674728792459542No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCA5590.2259389196967002No Hit
GGACTACTGGGGTATCTAATCCGGTTCGCTCCCCACGCTTTCGTGCCTTA5550.22432218324090988No Hit
GGACTACTCGGGTATCTAATCCTGTTCGCTCCCCCGGCTTTCGCACTTCA5510.22270544678511958No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCA5490.22189707855722438No Hit
GGACTACCAGGGTATCTAATCCGGTTCGCTCCCCACGCTTTCGTGCCTTA5370.2170468691898534No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5250.21219665982248231No Hit
GGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCA5190.20977155513879683No Hit
GGACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCA5170.20896318691090168No Hit
GGACTACAAGGGTATCTAATCCGGTTCGCTCCCCACGCTTTCGTGCCTTA5170.20896318691090168No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA5030.2033046093156355No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCA5010.20249624108774028No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA4980.20128368874589753No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA4890.19764603172036926No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4800.194008374694841No Hit
GGACTACCAGGGTTTCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCA4620.18673306064378445No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGAGCCTCA4620.18673306064378445No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCA4560.18430795596009894No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCCTTCGAGCCTCA4520.1826912195043086No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA4500.18188285127641343No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCA4490.18147866716246586No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCA4460.18026611482062307No Hit
GGACTACAGGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTAA4410.17824519425088517No Hit
GGACTACAGGGGTATCTAATCCGGTTCGCTCCCCACGCTTTCGTGCCTTA4380.17703264190904242No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA4380.17703264190904242No Hit
GGACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA4350.17582008956719966No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTCA4120.16652385494640518No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA4060.1640987502627197No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3980.16086527735113898No Hit
GGACTACAGGGGTATCTAATCCTGTTCGCTCCCCCGGCTTTCGCACTTCA3930.15884435678140105No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGA3880.15682343621166314No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3850.1556108838698204No Hit
GGACTACTAGGGTATCTAATCCTGTTCGATACCCGCACCTTCGTGCTTAA3820.15439833152797763No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA3790.15318577918613485No Hit
GGACTACAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3740.15116485861639695No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3580.14469791279323557No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCA3530.14267699222349767No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCCGGCTTTCGCACTTCA3340.13499749405849354No Hit
GGACTACCGGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTAA3280.132572389374808No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA3190.12893473234927974No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA3170.12812636412138456No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCCGGCTTTCGCACTTCA3160.127722180007437No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCACTTCA3150.1273179958934894No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCCGGCTTTCGCACTTCA3130.12650962766559423No Hit
GGACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA3080.12448870709585631No Hit
GGACTACTCGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTC3070.12408452298190871No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCCTTCGAGCCTCA2960.11963849772848528No Hit
GGACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCA2950.11923431361453769No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGA2950.11923431361453769No Hit
GGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCA2920.11802176127269494No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA2910.11761757715874736No Hit
GGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGAGCCTCA2860.11559665658900943No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCA2790.11276736779137633No Hit
GACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG2770.11195899956348117No Hit
GGACTACCGGGGTATCTAATCCTGTTCGCTCCCCCGGCTTTCGCACTTCA2760.11155481544953356No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGAGCCTCA2760.11155481544953356No Hit
GGACTACCCGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTAA2760.11155481544953356No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2740.11074644722163841No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCA2690.10872552665190047No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2660.10751297431005771No Hit
GACTACTAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG2650.10710879019611014No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA2630.10630042196821496No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA2620.10589623785426738No Hit
GGACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA2620.10589623785426738No Hit
GGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACCTTCGAGCTTAA2550.10306694905663429No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTCA2540.1026627649426867No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGA2520.10185439671479152No Hit
GGACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA2480.10023766025900119No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTGAG50.00103637662808.435295
AATTTGT50.00103637662808.435295
ATGTGAA50.00103637662808.435295
ATTGTCG50.00103637662808.435295
GTTAGCG50.00103637662808.435295
TATGCTA50.00103637662808.435295
TTTGGCG109.79966E-72808.435295
AGTAAGG50.00103637662808.435295
GTAGCTT153.3065044E-61872.2902295
AATTAGT351.2732926E-111604.82295
AGTTAGG900.01560.2417295
TTTGTGA100.0041445261404.2175295
TTAGACG207.835561E-61404.2175295
CGGTTAT100.0041445261404.2175295
GTTAGGG100.0041445261404.2175295
ATGTCAT100.0041445261404.2175295
ATGTAGG100.0041445261404.2175295
GTGCTAA100.0041445261404.2175295
AATTGAT207.835561E-61404.2175295
CAAGGTG100.0041445261404.2175295
Produced by FastQC (version 0.11.5)