Number raw input seqs 323637 Length outside bounds of 200 and 1000 30130 Num ambiguous bases exceeds limit of 6 5 Missing Qual Score 0 Mean qual score below minimum of 25 2028 Max homopolymer run exceeds limit of 6 7392 Num mismatches in primer exceeds limit of 0: 284082 Sequence length details for all sequences passing quality filters: No sequences passed quality filters for writing. Barcodes corrected/not 0/0 Uncorrected barcodes will not be written to the output fasta file. Corrected barcodes will be written with the appropriate barcode category. Corrected but unassigned sequences will not be written unless --retain_unassigned_reads is enabled. Total valid barcodes that are not in mapping file 0 Sequences associated with valid barcodes that are not in the mapping file will not be written. Barcodes in mapping file Sample Sequence Count Barcode C2A 0 TGTACTACTC B1B 0 TGATACGTCT B3A 0 TCTCTATGCG C1A 0 TCTACGTAGC C2C 0 TCGTCGCTCG B2C 0 TCACGTACTA C1C 0 TAGACTGCAC C3B 0 TACTCTCGTG C2B 0 TACGAGTATG B3B 0 CTGTACATAC B2B 0 CTCTACGCTC B2A 0 CTCGCGTGTC B1A 0 CGTGTCTCTA B3C 0 CGTCTAGTAC C1B 0 CGTAGACTAG N5 0 ATCAGACACG N6 0 ATATCGCGAG B1C 0 ATACGACGTA N4 0 AGCACTGTAG N3 0 AGACGCACTC N2 0 ACGCTCGACA N1 0 ACGAGTGCGT C3A 0 ACGACTACAG C3C 0 ACATACGCGT Total number seqs written 0