Input file paths
Mapping filepath: /home/qiime/Desktop/Shared_Folder/Sachia/MESOAQUA/mapping_files/validate_mapping_file_output/mappingfile_MESOAQUA_DNA_new_corrected.txt (md5: f9cddcc5a0c14da6c318c3b6bcd2f1bf)
Sequence read filepath: /home/qiime/Desktop/Shared_Folder/Sachia/MESOAQUA/barcode_trim/third_trim_stitched_reads_DNA/reads_DNA.fastq (md5: 2a46aefda261d6ba49632cf1a47150ed)
Barcode read filepath: /home/qiime/Desktop/Shared_Folder/Sachia/MESOAQUA/barcode_trim/second_trim_stitched_reads_DNA/barcodes_combined_DNA.fastq (md5: c3a1ba89129c1452d2108e5a546f9dbd)

Quality filter results
Total number of input sequences: 5360574
Barcode not in mapping file: 4791072
Read too short after quality truncation: 569461
Count of N characters exceeds limit: 0
Illumina quality digit = 0: 0
Barcode errors exceed max: 0

Result summary (after quality filtering)
Median sequence length: 316.00
MD3.1	3
MD25.5	3
MD25.3	2
MD17.12	2
MD0.7	2
MD5.6	1
MD5.2	1
MD3.9	1
MD3.6	1
MD3.4	1
MD3.3	1
MD3.12	1
MD3.11	1
MD3.10	1
MD25.7	1
MD25.2	1
MD25.10	1
MD21.8	1
MD21.5	1
MD21.4	1
MD21.12	1
MD21.11	1
MD17.3	1
MD17.11	1
MD17.10	1
MD10.9	1
MD10.8	1
MD10.5	1
MD10.3	1
MD10.11	1
MD0.9	1
MD0.8	1
MD0.11	1
MD0.1	1
MD5.9	0
MD5.8	0
MD5.7	0
MD5.5	0
MD5.4	0
MD5.3	0
MD5.12	0
MD5.11	0
MD5.10	0
MD5.1	0
MD3.8	0
MD3.7	0
MD3.5	0
MD3.2	0
MD25.9	0
MD25.8	0
MD25.6	0
MD25.4	0
MD25.12	0
MD25.11	0
MD25.1	0
MD21.9	0
MD21.7	0
MD21.6	0
MD21.3	0
MD21.2	0
MD21.10	0
MD21.1	0
MD17.9	0
MD17.8	0
MD17.7	0
MD17.6	0
MD17.5	0
MD17.4	0
MD17.2	0
MD17.1	0
MD10.7	0
MD10.6	0
MD10.4	0
MD10.2	0
MD10.12	0
MD10.10	0
MD10.1	0
MD0.6	0
MD0.5	0
MD0.4	0
MD0.3	0
MD0.2	0
MD0.12	0
MD0.10	0

Total number seqs written	41
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Input file paths
Mapping filepath: /home/qiime/Desktop/Shared_Folder/Sachia/MESOAQUA/mapping_files/validate_mapping_file_output/mappingfile_MESOAQUA_RNA_FR_corrected.txt (md5: 8831c70d3d51f9bccfa89911a51ea4fd)
Sequence read filepath: /home/qiime/Desktop/Shared_Folder/Sachia/MESOAQUA/barcode_trim/third_trim_stitched_reads_RNA/reads_RNA.fastq (md5: 8f3e6eea759dbef1e6a43fb6f859af64)
Barcode read filepath: /home/qiime/Desktop/Shared_Folder/Sachia/MESOAQUA/barcode_trim/second_trim_stitched_reads_RNA/barcodes_combined_RNA.fastq (md5: 94fe5da4741c0e24d2284423b81d84e5)

Quality filter results
Total number of input sequences: 12672422
Barcode not in mapping file: 11032874
Read too short after quality truncation: 8848
Count of N characters exceeds limit: 90
Illumina quality digit = 0: 0
Barcode errors exceed max: 0

Result summary (after quality filtering)
Median sequence length: 252.00
MR3.12	30308
MR7.03	28668
MR3.08	27292
MR3.06	25700
MR3.04	25600
MR25.04	25230
MR7.01	25086
MR7.04	25005
MR17.04	24820
MR10.04	24694
MR10.12	24155
MR7.07	24150
MR3.03	24131
MR0.03	24097
MR3.01	23771
MR0.10	23348
MR3.07	23252
MR7.08	23234
MR21.03	23078
MR3.05	23058
MR21.06	23007
MR0.08	23001
MR25.12	22892
MR10.10	22712
MR17.01	22689
MR0.12	22645
MR0.02	22586
MR10.02	22164
MR17.12	21949
MR3.02	21875
MR17.11	21765
MR17.08	21649
MR21.11	21544
MR0.11	21529
MR0.07	21467
MR25.05	21308
MR7.10	21286
MR21.02	21257
MR25.01	21124
MR17.07	21070
MR7.02	20810
MR0.05	20778
MR10.01	20764
MR7.12	20754
MR10.03	20580
MR25.03	20245
MR17.02	20164
MR0.09	20140
MR21.01	19792
MR21.08	19791
MR25.11	19780
MR10.07	19720
MR7.06	19179
MR7.05	19109
MR21.10	18945
MR21.05	18831
MR25.08	18761
MR10.08	18713
MR10.09	18670
MR17.03	18589
MR17.09	18483
MR21.12	18129
MR17.10	17995
MR3.09	17985
MR3.11	17484
MR21.09	17295
MR21.07	17194
MR10.11	16404
MR7.09	16204
MR0.01	16171
MR0.04	15879
MR0.06	15075
MR7.11	14738
MR25.02	14162
MR10.06	13571
MR17.06	13058
MR10.05	11485
MR25.06	10236
MR25.09	8807
MR25.07	4909
MR3.10	3035

Total number seqs written	1630610
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